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Still glowing after all these years: storage life ofa complete bovine sexing pcr mix.

Reproduction, Fertility and Development.. 2008-01;  20(1):215-215
West R., Valentin C., Herr C.
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摘要

generally, PCR reaction mixes are made fresh on the day of use. This is impractical for field sexing. Mistakes occur due to the required measuring precision and to contamination from environmental DNA. Therefore, we developed a complete reaction mix (YCD). Storage and transport of YCD required liquid N2. No study has determined storage life of PCR reaction mixes in standard freezers. Our study compared 14-year-old bovine YCD (AB Technology, Pullman, WA, USA) stored in a standard freezer to a freshly prepared mix. The working concentration of bovineYCD was: buffer (50 mm Tris, 1% dextran T-500, 50 mm KCl, 2.5 mm MgCl... More

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